UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto.

UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.

www.uhn.ca

Union: Non-Union 
Number of Vacancies: 1
Site: Princess Margaret Cancer Centre
Department: Research
Reports to: Principal Investigator
Hours: 37.5 Per Week
Shifts: Day Shift
Status: Permanent Full-Time
Closing Date: November 7, 2025

Job Description:
We are seeking a highly motivated and detail-oriented Laboratory Technician to join our research team. The successful candidate will play a key role in establishing and maintaining workflows for protein production and purification across multiple expression systems, including bacterial, fungal, insect, and mammalian cells.

Position Summary:
This is an exciting opportunity to contribute to cutting-edge research in protein science within a collaborative and dynamic environment. You will gain experience across diverse expression systems and be part of a team working toward understanding and targeting complex biological systems.

Duties:

• Establish and optimize protein expression systems in various hosts (E. coli, yeast, insect, and mammalian cells).
• Perform protein purification using affinity, ion exchange, and size-exclusion chromatography.
• Maintain laboratory records, prepare reagents, and ensure proper equipment maintenance.
• Collaborate with students/staff to support structural, biochemical, and functional studies of recombinant proteins.
• Contribute to troubleshooting and optimization of purification protocols for yield and quality.

• Master's degree in Biochemistry, Molecular Biology, Biotechnology, or a related discipline.
• Hands-on experience in protein expression and purification.
• Familiarity with AKTA or equivalent chromatography systems is an asset.
• Excellent organizational and communication skills.
• Ability to work both independently and collaboratively in a multidisciplinary team.
• Expertise in membrane protein purification is a strong plus.

Why join UHN?

In addition to working alongside some of the most talented and inspiring healthcare professionals in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.

• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/)
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

All applications must be submitted before the posting close date.

UHN uses email to communicate with selected candidates.  Please ensure you check your email regularly.

Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

We thank all applicants for their interest, however, only those selected for further consideration will be contacted.